ELISA PROTOCOL(Yale University)文章来源:工业电气网 作者:【未知】 点击:次 时间:2016/11/29 4:27:51[ELISA PROTOCOL(Yale University)]:1. Materials(1) TS Buffer: 10 mM Tris pH 8.0 150 mM NaCl(2) TST Buffer: TS Buffer with 0.05% Tween-2...nandwash3XwithTST.

(7)Add50 lperwellofthep-nitrophenylphosphatesolutionandincubateatroomtemperaturefor10-30min.Stopthereactionsbyadding50 lof0.1MEDTApH8.0.Forscreeningmonoclonalsitisbesttoletthemgountilallofthepositivesareclearlyvisible,howeverifyouwanttobequantitativeitisimportantnottoletthemgetintenselyyellowortheywillbeoffscale--theplatereaderismostaccuratewhenthewellshavejustbeguntoclearlyturnyellow.

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